Journal: Cell communication and signaling : CCS

Article Title: VDR promotes pancreatic cancer progression in vivo by activating CCL20-mediated M2 polarization of tumor associated macrophage.

doi: 10.1186/s12964-024-01578-x

Figure Lengend Snippet: Fig. 1 Elevated expression of VDR in PAAD. (A) The level of VDR expression in different tumor types from TCGA and GTEx databases. (B-C) OS and DFI of VDR high and low PAAD patients analyzed by TCGA data. (D-E) Univariate and Multivariate Cox analysis showing the hazard ratios (HRs) of different factors. (F-G) The relative expression of VDR in 19 cases of PAAD and paired paracancerous tissues was detected using immunofluorescence. Scale bars = 50 μm. (P < 0.05). (H-I) The expression of VDR and CCL20 was compared among different PAAD cell lines. The values in the graphs represent the mean ± SD. * means P < 0.05; ** means P < 0.01, *** means P < 0.001

Article Snippet: Cell culture and viral infection Human pancreatic ductal epithelial HPDE6-C7 and human PAAD cell lines (PL-45, Capan1, BxPC3, PSN1, Miapaca2, Panc1, SW1990) were purchased from American Type Culture Collection.

Techniques: Expressing, Immunofluorescence

Journal: Cell communication and signaling : CCS

Article Title: VDR promotes pancreatic cancer progression in vivo by activating CCL20-mediated M2 polarization of tumor associated macrophage.

doi: 10.1186/s12964-024-01578-x

Figure Lengend Snippet: Fig. 3 VDR overexpression tumor cells promote the macrophages polarization and recruitment. (A) Construction of recombinant cell lines overexpress ing and knocking down VDR was achieved using lentivirus in Panc1 and Capan1, respectively. (B-G) qPCR, immunofluorescence, and western blot were performed to measure the expression of M1 and M2 markers after co-culturing with transfected PAAD cells. Scale bars = 50 μm. (H-I) Representative imag es of macrophage migration assays and quantitative chart. Scale bars = 200 μm. (J) mIHC was performed on PAAD tumors from TMA. Scale bars = 100 μm. The values on the graphs represent are mean ± SD. Statistical significance was determined as *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: Cell culture and viral infection Human pancreatic ductal epithelial HPDE6-C7 and human PAAD cell lines (PL-45, Capan1, BxPC3, PSN1, Miapaca2, Panc1, SW1990) were purchased from American Type Culture Collection.

Techniques: Over Expression, Recombinant, Immunofluorescence, Western Blot, Expressing, Transfection, Migration

Journal: Cell communication and signaling : CCS

Article Title: VDR promotes pancreatic cancer progression in vivo by activating CCL20-mediated M2 polarization of tumor associated macrophage.

doi: 10.1186/s12964-024-01578-x

Figure Lengend Snippet: Fig. 4 VDR promotes the transcription and release of CCL20. (A) Heat map displayed the correlation between VDR and macrophage M2 polarization and recruitment gene set in TCGA PAAD data set. (B) Gene expression validation by qPCR. (C) The expression of CCL20 between normal and PAAD tis sues. (D-E) Kaplan-Meier curves for CCL20 high and low groups. (F-G) The expressions of VDR and CCL20 detected by western blot. (H) Measurement of CCL20 concentration in the supernatants of VDR overexpression and VDR knockdown tumor cells by ELISA. (I) Prediction of VDR and CCL20 binding sites. (J) ChIP assay confirmed that VDR could bind to the CCL20 promoter in Panc1. (K) Schematic representation of the CCL20 promoter and its mutant site luciferin-reporter gene. (L) Luciferase assay demonstrated that VDR promoted CCL20 transcription. Values in the graphs are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: Cell culture and viral infection Human pancreatic ductal epithelial HPDE6-C7 and human PAAD cell lines (PL-45, Capan1, BxPC3, PSN1, Miapaca2, Panc1, SW1990) were purchased from American Type Culture Collection.

Techniques: Gene Expression, Biomarker Discovery, Expressing, Western Blot, Concentration Assay, Over Expression, Knockdown, Enzyme-linked Immunosorbent Assay, Binding Assay, Mutagenesis, Luciferase